Using bimetallic Au@ Pt nanozymes as a visual tag and as an enzyme mimic in enhanced sensitive lateral-flow immunoassays: Application for the detection of streptomycin

References

ref title DOI material type comment
3390 159 Using bimetallic Au@ Pt nanozymes as a visual tag and as an enzyme mimic in enhanced sensitive lateral-flow immunoassays: Application for the detection of streptomycin https://doi.org/10.1016/j.aca.2020.06.009 Multi-metal Au@Pt

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7452 159 Au@Pt 50 nm TEM A typical TEM image (Fig. 3) showed that the Au@Pt nanozymes were relatively uniform in size and similar in structure, and the diameter of the nanoparticles was approximately 50 nm. As a uniform porous structure, Pt NPs formed a branched structure on the surface ofAu. The particle size ofthe Au corewas approximately 30e35 nm, and the average diameter of the Pt NPs was approximately 5e10 nm.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6044 159 HRP POD TMB 0.3296 mM 7.638 -8 M/s 5.894 1/s 159
6043 159 Au@Pt POD TMB 2.431 -3 mM 4.425 -8 M/s 1.475 3 1/s 159
6042 159 HRP POD H2O2 1.468 mM 22.88 -8 M/s 18.3 1/s 159
6041 159 Au@Pt POD H2O2 4.076 -3 mM 6.013 -8 M/s 2.004 3 1/s 159 The apparent Km values for Au@Pt nanozyme were about 80e600 times smaller than that ofHRP, which indicated that the affinity between TMB/H2O2 and Au@Pt nanozyme was stronger than that between HRP and TMB/H2O2. Moreover, the Kcat values for Au@Pt nanozyme were about 80e340 times higher than that of HRP, which indicated that the catalytic efficiency of Au@Pt nanozyme was stronger than that of HRP

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5065 159 Au@Pt Au@Pt nanozymes were introduced to develop a low-cost, rapid, visual and highly sensitive immunochromatographic assay for streptomycin detection streptomycin Color 0.06 ng/ml The qualitative LOD was 0.1 ng mL−1 by the naked eye, and the quantitative LOD was 0.06 ng mL−1.
5064 159 Au@Pt Au@Pt nanozymes were introduced to develop a low-cost, rapid, visual and highly sensitive immunochromatographic assay for streptomycin detection streptomycin Color 0.06 ng/ml