White Peroxidase‐Mimicking Nanozymes: Colorimetric Pesticide Assay without Interferences of O2 and Color

References

ref title DOI material type comment
3431 200 White Peroxidase‐Mimicking Nanozymes: Colorimetric Pesticide Assay without Interferences of O2 and Color https://doi.org/10.1002/adfm.202001933 Metal oxide GeO2

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7486 200 GeO2 showed besom-like morphology with uniform size (width of ≈100 nm and length of ≈1 µm). the “head of besom” was composed of long strip with width of ≈10 nm (Figure 1c,d).

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6097 200 GeO2 POD H2O2 1.75 mM 23.400 -8 M/s 200 200
6096 200 GeO2 POD TMB 0.42 mM 23.397 -8 M/s 200

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5111 200 GeO2 Colorimetric Assay of OPs paraoxon Color 0.1-50 pM 14 fM
5110 200 GeO2 Colorimetric Assay of OPs paraoxon Color 0.1-50 pM 14 fM Typically, 100 µL of different concentrations of paraoxon (0, 0.1, 2, 5, 10, 15, 30, 50, 70, 100 pm) were mixed with 20 µL of PB solutions (0.1 m, pH 8.0) containing AChE (10 µg mL−1). After the incubation for 20 min at 37 °C, 20 µL of ATCh solution (10 mm) and 20 µL of GeO2 nanozymes solution (1 mg mL−1) were added into the above mixture respectively for another 20 min incubation. The residual GeO2 nanozymes was collected by centrifugation, and added into 200 µL of acetate buffer (pH 4.0, 0.1 m) containing TMB (0.6 mm) and H2O2 (1.2 mm). Finally, the absorbance of the above reaction was measured after 30 min. Each experiment was repeated three times. The LOD was calculated by the equation LOD = (3σ/s), where σ is the standard deviation of blank signals and s is the slope of the calibration curve.