Aptamer-gold nanozyme based competitive lateral flow assay for rapid detection of CA125 in human serum

References

ref title DOI material type comment
3555 362 Aptamer-gold nanozyme based competitive lateral flow assay for rapid detection of CA125 in human serum https://doi.org/10.1016/j.bios.2020.112368 Metal Aptamer-gold nanozyme

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7601 362 Aptamer-gold nanozyme 21 nm DLS the average diameter of AuNPs to be ~10 nm .The absorption maxima of AuNPs after conjugation shifted from 521 nm to 530 nm (Fig. 2A) and particle size increases from 10 nm to 21 nm was observed (Fig. 2B and C).

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6240 362 AuNPs POD TMB 8.7719 μM/min 3364 1/min 362

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5264 362 Aptamer-gold nanozyme to develop an Aptamer-nanozyme lateral flow assay (ALFA) CA125 in human serum CL 7.5-200 U/mL 5.21 U/mL