Urchin peroxidase-mimicking Au@ Pt nanoparticles as a label in lateral flow immunoassay: impact of nanoparticle composition on detection limit of Clavibacter michiganensis

References

ref title DOI material type comment
3573 385 Urchin peroxidase-mimicking Au@ Pt nanoparticles as a label in lateral flow immunoassay: impact of nanoparticle composition on detection limit of Clavibacter michiganensis https://doi.org/10.1007/s00604-020-04253-3 Multi-metal Au@Pt nanoparticles

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7623 385 Au@Pt nanoparticles 20-2000 nm TEM The size of the synthesized GNPs according to TEM was 20.0 ± 2.6 nm (n = 100, Fig. 2a). 4.4 U/mg These changes led to a 70-fold increase in peroxidase-mimicking activity in the solution (specific activity 0.06–4.4 U mg−1) and a 30-fold decrease in LOD using the catalytic activity of Au@Pt.
7622 385 Au@Pt nanoparticles 20 2.6 nm TEM The size of the synthesized GNPs according to TEM was 20.0 ± 2.6 nm (n = 100, Fig. 2a). 4.4 U/mg These changes led to a 70-fold increase in peroxidase-mimicking activity in the solution (specific activity 0.06–4.4 U mg−1) and a 30-fold decrease in LOD using the catalytic activity of Au@Pt.
7621 385 Au@Pt nanoparticles 20 2.6 nm TEM The size of the synthesized GNPs according to TEM was 20.0 ± 2.6 nm (n = 100, Fig. 2a). 4 U/mg
7620 385 Au@Pt nanoparticles 20-2000 nm TEM The size of the synthesized GNPs according to TEM was 20.0 ± 2.6 nm (n = 100, Fig. 2a). 4 U/mg

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6268 385 Au@Pt nanoparticles POD 385

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5293 385 Au@Pt nanoparticles a signal amplification strategy a widespread and dangerous phytopathogenic bacteria species (Clavibacter michiganensis)