Colorimetric determination of tumor cells via peroxidase-like activity of a cell internalizable nanozyme: Hyaluronic acid attached-silica microspheres containing accessible magnetite nanoparticles

References

ref title DOI material type comment
3632 444 Colorimetric determination of tumor cells via peroxidase-like activity of a cell internalizable nanozyme: Hyaluronic acid attached-silica microspheres containing accessible magnetite nanoparticles https://doi.org/10.1016/j.colsurfa.2020.124812 Composite HA attached form of Fe3O4@SiO2 microspheres (HA@Fe3O4@SiO2 microspheres)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7672 444 HA@Fe3O4@SiO2 6.24 μm SEM
7671 444 Fe3O4@SiO2 6.25 μm SEM

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5357 444 HA@Fe3O4@SiO2 Colorimetric determination of tumor cells tumor cell Color In the presence of HeLa cells, the visible region absorbance sharply decreased up to the cell concentration of 0.25 × 106 cells/mL, with both nanozymes (Fig. 9A). In the concentration range of 0.25–4.0 × 106 cells/mL, the visible region absorbance linearly decreased with the increasing cell concentration, with satisfactorily high correlation coefficients for both nanozymes. However, a sharper linear decrease with almost 1.6 times higher slope was observed with HA@Fe3O4@SiO2 microspheres with respect to Fe3O4@SiO2 microspheres with HeLa cells.
5356 444 HA@Fe3O4@SiO2 Colorimetric determination of tumor cells tumor cell Color