Highly sensitive and simple colorimetric assay of hydrogen peroxide and glucose in human serum via the smart synergistic catalytic mechanism

References

ref title DOI material type comment
3640 452 Highly sensitive and simple colorimetric assay of hydrogen peroxide and glucose in human serum via the smart synergistic catalytic mechanism https://doi.org/10.1016/j.saa.2020.118233 Composite graphene oxide (GO)/AuNPs nanocomposite

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7688 452 GO/AuNPs 31 nm TEM TEM results (see the Supporting Information, Fig. S1) showed that spherical nanoparticles were uniformly dispersed and embedded on the surface of GO and the particle size of nanoparticles was about 31 nm.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5370 452 GO/AuNPs H2O2 detection H2O2 Color 3.8×10–7~5.5×10–5 M 4.2×10–8 M
5369 452 GO/AuNPs glucose detection glucose Color 5.1×10–6~5.1×10–4 M 6.3×10–7 M 105.3%-108.3%
5368 452 GO/AuNPs glucose detection glucose Color 5.1×10–6~5.1×10–4 M 6.3×10–7 M 105.3%-108.3% The real human serum was used as test samples to investigate the practical application of the proposed analysis. Human serum samples were taken from Xi'a University of Science and Technology Hospital. As the normal content of human blood glucose is 3.9–6.1 mmol/L [38], before the test, the collected serum samples need to be diluted to meet the requirements of this method after centrifugation. The glucose test results are shown in Table 2. For three different test samples, the detection results from this method are not substantially different from those given by the hospital.