Enhancement of gold nanoclusters-based peroxidase nanozymes for detection of tetracycline

References

ref title DOI material type comment
3854 668 Enhancement of gold nanoclusters-based peroxidase nanozymes for detection of tetracycline https://doi.org/10.1016/j.microc.2020.104871 Metal In this study, we report a simple protocol for the synthesis of D-trytophan methyl ester protected AuNCs and exhibit their peroxidase-like activity in a chromogenic reaction coupling with 3,3′,5,5′-tetramethylbenzidine-H2O2.

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7885 668 D-Trp-OMe@AuNCs 2.3 nm DLS As shown in Fig. 2a and b, the D-Trp-OMe@AuNCs were monodisperse and spherical with an average size of 2.3 nm. The addition of TC induced the aggregation of D-Trp-OMe@AuNCs to form the D-Trp-OMe@AuNCs-TC composites [28] (Fig. 2d). Fig. 2c clearly shows that 24.2 nm was the average size of the D-Trp-OMe@AuNCs-TC composites.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6554 668 D-Trp-OMe@AuNCs-TC POD H2O2 0.01 1.748 -8 M/s 668
6553 668 D-Trp-OMe@AuNCs-TC POD TMB 0.254 1.923 -8 M/s 668
6552 668 D-Trp-OMe@AuNCs POD H2O2 0.057 0.831 -8 M/s 668
6551 668 D-Trp-OMe@AuNCs POD TMB 0.182 0.192 -8 M/s 668

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5598 668 D-Trp-OMe@AuNCs detection of trtracycline TMB Color 1.5-30.0 μM 0.20 μM 99.0-105.0%