Prussian blue nanoparticles with peroxidase-mimicking properties in a dual immunoassays for glycocholic acid

References

ref title DOI material type comment
3862 676 Prussian blue nanoparticles with peroxidase-mimicking properties in a dual immunoassays for glycocholic acid https://doi.org/10.1016/j.jpba.2020.113317 Others Prussian Blue nanoparticles (PBNPs) were utilized in a lateral flow immunoassay (LFA) and in an indirect competitive nanozyme-linked immunosorbent assay (icELISA), respectively, for their intense blue color and peroxidase (POx) -like activity.

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7893 676 PBNPs 150 26 nm SEM PBNPs was characterized by SEM to observe the morphology. As shown in Fig. 2A, the PBNPs were well-prepared and in the shape of cubes with an average diameter of 150 ± 26 nm.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6571 676 PBNPs POD 676

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5606 676 PBNPs-icELISA determination of free GCA GCA Color 0.03-1.20 μg/mL 2.5 × 10−3 μg/mL