Electrochemical detection of β-lactoglobulin based on a highly selective DNA aptamer and flower-like Au@ BiVO4 microspheres

References

ref title DOI material type comment
3869 683 Electrochemical detection of β-lactoglobulin based on a highly selective DNA aptamer and flower-like Au@ BiVO4 microspheres https://doi.org/10.1016/j.aca.2020.04.066 Composite The flower-like BiVO4 microspheres were firstly found to have peroxidase mimic catalytic activity and used to amplify the electrochemical signal. The aptamer can bind β-lactoglobulin and fall off from the working electrode, after which the DNA2/Au/BiVO4 probe can be fixed to the DNA1/AuNPs/ITO working electrode by the hybridization of DNA2 with DNA1.

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7899 683 BiVO4 nm SEM It is clear that the flower-like BiVO4 microspheres with uniform structure were assembled from BiVO4 nanosheets and were basically consistent with the description in the literature [26].

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6577 683 BiVO4 POD 683

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5612 683 BiVO4 detection of β-lactoglobulin β-lactoglobulin E-chem 0.01-1000 ng/mL 0.007 ng/mL