Incorporation of a Biocompatible Nanozyme in Cellular Antioxidant Enzyme Cascade Reverses Huntington's Like Disorder in Preclinical Model

References

ref title DOI material type comment
4024 838 Incorporation of a Biocompatible Nanozyme in Cellular Antioxidant Enzyme Cascade Reverses Huntington's Like Disorder in Preclinical Model https://doi.org/10.1002/adhm.202001736 Metal oxide citrate functionalized manganese-based biocompatible nanoscalematerial (C-Mn3O4 NPs)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
8036 838 C-Mn3O4 NPs 6.12 2.24 nm TEM Transmission electron micrograph (TEM) shows the C-Mn3O4 NPs to be well-dispersed uniform spheres with an average diameter of ≈6.12 ± 2.24 nm.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6737 838 C-Mn3O4 NPs GPx GSH 1.36 0.09 mM 0.064 0.008 mM/min 46.73 0.31 1/min 838 M-1 s-1 For oxidation of GSH, the values were found to be kcat ≈ 46.73 ± 0.31 min−1 and kcat/KM ≈ 5717.85 ± 345.72 M s−1.
6736 838 C-Mn3O4 NPs GPx H2O2 1.09 0.06 mM 0.095 0.011 mM/min 69.12 0.52 1/min 838 M-1 s-1 The C-Mn3O4 nanozyme catalyzed the reduction of H2O2 with a turnover number (kcat) of ≈69.12 ± 0.52 min−1 and an apparent second-order rate constant, kcat/KM ≈ 10 530.17 ± 975.25 M s−1.

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5766 838 C-Mn3O4 NPs cellular antioxidant enzyme cascade reverses huntington's like disorder in preclinical model