Colorimetric biosensing of nopaline synthase terminator using Fe3O4@ Au and hemin-functionalized reduced graphene oxide

References

ref title DOI material type comment
4043 857 Colorimetric biosensing of nopaline synthase terminator using Fe3O4@ Au and hemin-functionalized reduced graphene oxide https://doi.org/10.1016/j.ab.2020.113798 Composite gold nanoparticles doped magnetic Fe3O4 nanoparticles (Fe3O4@Au NP), capture probe DNA (cDNA), and hemin-functionalized reduced graphene oxide nanosheets (H-GN)(Fe3O4@Au@cDNA@H-GN)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
8051 857 Fe3O4@Au@cDNA@H-GN 250 125 nm SEM The particle size of the Fe3O4 MNPs ranges from 125 to 375 nm. It is obvious that Au NPs (~4 nm, Fig. 1C inset) attach and distribute densely on the surface of Fe3O4 NPs.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6748 857 Fe3O4@Au@cDNA@H-GN POD TMB + H2O2 857

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5785 857 Fe3O4@Au@cDNA@H-GN colorimetric biosensing of nopaline synthase terminator nopaline synthase terminator Color 0.5-100 nM 0.19 nM