A colorimetric immunoassay for determination of Escherichia coli O157: H7 based on oxidase-like activity of cobalt-based zeolitic imidazolate framework

References

ref title DOI material type comment
4509 1347 A colorimetric immunoassay for determination of Escherichia coli O157: H7 based on oxidase-like activity of cobalt-based zeolitic imidazolate framework https://doi.org/10.1007/s00604-020-04407-3 Composite Preparation of the immunoprobe involved self-polymerized dopamine being applied for the surface modification of ZIF-67 nanosheets in order to bind to the antibody(ZIF-67@PDA@antibody)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
8354 1347 Fe3O4@PDA 40 nm TEM
8353 1347 Fe3O4@PDA 100 nm SEM
8352 1347 ZIF-67 nanosheets 400 nm SEM The morphology of ZIF-67 nanosheets shows irregular flakes that are 400 nm in diameter. Modification of dopamine on the surface of ZIF-67 nanosheets did not change its morphology.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
6197 1347 ZIF-67@PDA determination of Escherichia coli O157:H7 Escherichia coli O157:H7 Color 30-3.0×10^8 CFU/mL 12 CFU/mL