A sandwich ELISA-like detection of C-reactive protein in blood by citicoline-bovine serum albumin conjugate and aptamer-functionalized gold nanoparticles nanozyme

References

ref title DOI material type comment
3425 194 A sandwich ELISA-like detection of C-reactive protein in blood by citicoline-bovine serum albumin conjugate and aptamer-functionalized gold nanoparticles nanozyme https://doi.org/10.1016/j.talanta.2020.121070 Composite aptamer-functionalized gold nanoparticles (aptamer-AuNPs)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7479 194 aptamer-AuNPs 18 nm DLS DLS is an effective method to measure the overall particle size distribution of nanomaterials. As can be seen from Fig. 3, the particle size of aptamer-AuNPs is about 18 nm with a small distribution range.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6085 194 aptamer-AuNPs 194 194

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5106 194 aptamer-AuNPs determination of CRP in blood C-reactive protein (CRP) Color 0.1-200 ng/mL 8 pg/mL 94.54%–98.03%