A Heparinase Sensor Based on a Ternary System of Hg2+–Heparin–Osmium Nanoparticles

References

ref title DOI material type comment
3453 222 A Heparinase Sensor Based on a Ternary System of Hg2+–Heparin–Osmium Nanoparticles https://doi.org/10.1021/acs.analchem.9b05222 Composite Hg2+–heparin–osmium nanoparticles (OsNPs)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7502 222 Hg2+/heparin–OsNPs 80.97 U/g TEM image of heparin–OsNPs. Inset: HRTEM image and the size distribution of heparin–OsNPs determined from the TEM image (from size distribution analysis of 50 random nanoparticles by Gaussian fitting).
7501 222 Hg2+/heparin–OsNPs 81 U/g

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6122 222 Hg2+/heparin–OsNPs OXD TMB 0.051 mM 3.28 -7 M/s 222 Hg2+ 40μM
6121 222 Hg2+/heparin–OsNPs OXD TMB 0.8 mM 8.45 -8 M/s 222 Hg2+ 0μM
6120 222 Hg2+/heparin–OsNPs OXD TMB 0.176 mM 1.52 -7 M/s 222 222

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5134 222 Hg2+/heparin–OsNPs detection of heparinase in human serum samples heparinase Color 20-1000 μg L-1 15 μg L-1