Nanozyme and aptamer-based immunosorbent assay for aflatoxin B1

References

ref title DOI material type comment
3612 424 Nanozyme and aptamer-based immunosorbent assay for aflatoxin B1 https://doi.org/10.1016/j.jhazmat.2020.123154 Composite mesoporous SiO2/Au-Pt (m-SAP)/cDNA

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7656 424 m-SAP/cDNA 220.82 nm TEM As exhibited in Fig. 3C, the hydrodynamic diameters of m-SiO2 NP, MNP-aptamer and m-SAP/c-DNA are 201.09, 117.95, 220.82 nm, respectively. When MNP-aptamer and m-SAP/cDNA incubated and reacted, the m-SAP/MNP complex generated and showed a large size increase to 457.43 nm, implying the successful hybridization of aptamer and cDNA.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6303 424 m-SAP/cDNA CAT H2O2 0.016 mM 1.473 -8 M/s 424
6302 424 m-SAP/cDNA CAT TMB 0.058 mM 1.279 -8 M/s 424

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5329 424 m-SAP/cDNA detection of aflatoxin B1 (AFB1) Aflatoxin B1 Color 0.01-1000 ng/ml 5 pg/ml 0.042000000000000003