Investigation of efficient synergistic and protective effects of chitosan on copper nanoclusters: Construction of highly active and stable nanozyme for colorimetric and fluorometric dual-signal biosensing

References

ref title DOI material type comment
4185 999 Investigation of efficient synergistic and protective effects of chitosan on copper nanoclusters: Construction of highly active and stable nanozyme for colorimetric and fluorometric dual-signal biosensing https://doi.org/10.1016/j.snb.2021.129522 Composite Copper nanoclusters (CuNCs) have advantages of low cost, excellent biocompatibility, and good fluorescence properties, but they are seldom used as nanozymes for biosensing owing to their low catalytic activity and poor stability. Herein, a natural polymer, chitosan (CS), was explored to effectively promote the peroxidase-like property of glutathione-protected copper nanoclusters (GSH-CuNCs).

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6921 999 CS@GSH-CuNCs POD H2O2 27.3 mM 2.53 -7 M/s 999
6916 999 CS@GSH-CuNCs POD ABTS 1.67 mM 4.85 -7 M/s 999

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5909 999 CS@GSH-CuNCs Fluor 5-100 mU/mL 1.4 mU/mL
5908 999 CS@GSH-CuNCs detection of AChE activity. Color 10-120 mU/mL 3.3 mU/mL
5907 999 CS@GSH-CuNCs detection of choline Fluor 5-100 μM 1.2 μM
5906 999 CS@GSH-CuNCs detection of choline Color 20-150 μM 6.5 μM