Colorimetric biosensing of nopaline synthase terminator using Fe3O4@ Au and hemin-functionalized reduced graphene oxide

References

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title
DOI
material type
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857Colorimetric biosensing of nopaline synthase terminator using Fe3O4@ Au and hemin-functionalized reduced graphene oxidehttps://doi.org/10.1016/j.ab.2020.113798Compositegold nanoparticles doped magnetic Fe3O4 nanoparticles (Fe3O4@Au NP), capture probe DNA (cDNA), and hemin-functionalized reduced graphene oxide nanosheets (H-GN)(Fe3O4@Au@cDNA@H-GN)

Materials

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material
size
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size type
size comment
BET
b nanozyme
b 10n
b unit
specific act
sa 10n
sa unit
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857Fe3O4@Au@cDNA@H-GN250125nmSEMThe particle size of the Fe3O4 MNPs ranges from 125 to 375 nm. It is obvious that Au NPs (~4 nm, Fig. 1C inset) attach and distribute densely on the surface of Fe3O4 NPs.

Kinetics

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substrate
pH
T
km
km err
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km unit
vmax
vmax err
vmax 10n
vmax unit
kcat
kcat err
kcat 10n
kcat unit
kcat/km
kcat/km 10n
kcat/km unit
comment
857Fe3O4@Au@cDNA@H-GNPODTMB + H2O2857

Applications

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material
application
target
method
linear range
linear ran unit
LOD
lod unit
recovery
comment
857Fe3O4@Au@cDNA@H-GNcolorimetric biosensing of nopaline synthase terminatornopaline synthase terminatorColor0.5-100nM0.19nM