A peroxidase-mimicking Zr-based MOF colorimetric sensing array to quantify and discriminate phosphorylated proteins

References

ref title DOI material type comment
3368 137 A peroxidase-mimicking Zr-based MOF colorimetric sensing array to quantify and discriminate phosphorylated proteins https://doi.org/10.1016/j.aca.2020.04.073 MOF Zr-based MOF

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7429 137 Zr-MOF 60 nm SEM As demonstrated by Fig. 1C, the synthesized Zr-MOF presents a uniform spherical morphology, and the diameter is ∼60 nm. 217.5

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6011 137 Zr-MOF POD TMB 0.05 mM 137
6010 137 Zr-MOF POD H2O2 0.59 mM 137 137

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5036 137 Zr-MOF Quantification and discrimination of phosphorylated proteins α-casein Color 0.17-5 μg/mL 0.16 μg/mL Further, the absorbance at 652 nm is linearly decreased with the increased levels of α-CS ranging from 0.17 to 5.0 μg/mL (Fig. 4B). The equation can be written as A = −0.0554[α-CS] (μg/mL) + 0.4119 (R2 = 0.996). The limit of detection (LOD) is calculated to be 0.16 μg/mL based on S/N = 3.
5035 137 Zr-MOF Quantification and discrimination of phosphorylated proteins α-casein Color 0.17-5 μg/mL 0.16 μg/mL