Nanozyme Database

Carbon quantum dots originated from chicken blood as peroxidase mimics for colorimetric detection of biothiols

References

	ref	title	DOI	material type	comment
3397	166	Carbon quantum dots originated from chicken blood as peroxidase mimics for colorimetric detection of biothiols	https://doi.org/10.1016/j.jphotochem.2020.112529	Carbon	Carbon quantum dots (CQDs) from chicken blood

Materials

	ref	material	size	size err	size unit	size type	size comment	BET	b _nanozyme	b 10ⁿ	b unit	specific act	sa 10ⁿ	sa unit	comment
7458	166	CB-CQDs	1.5-3.6		nm	TEM	They exhibit a size range of 1.5–3.6 nm with an average diameter of about 2.4 nm, fitting well to the Gaussian function

Kinetics

	ref	material	enzyme type	substrate	pH	T	k_m	k_m err	k_m 10ⁿ	k_m unit	v_max	v_max err	v_max 10ⁿ	v_max unit	k_cat	k_cat err	k_cat 10ⁿ	k_cat unit	k_cat/k_m	k_cat/k_m 10ⁿ	k_cat/k_m unit	comment
6055	166	CB-CQDs	POD	TMB	4.5	RT	0.83			mM	5.13		-8	M/s					166			166
6054	166	CB-CQDs	POD	H2O2	4.5	RT	0.7			mM	4.09		-8	M/s					166

Applications

	ref	material	application	target	method	linear range	linear ran unit	LOD	lod unit	recovery	comment
5074	166	CB-CQDs	The detection of biothiols was performed as follows: in a series of colorimetric tubes, 0.5 mL of TMB (20 mM), 0.5 mL of H2O2 (25 mM), and 0.1 mL of CB-CQDs were fully mixed in 3.8 mL of HAc-NaAc buffer at pH4.5. Then, various concentrations of biothiols standard solution (0.1 mL) were added into the above mixture. After they were well mixed and incubated at 40 °C for 25 min, the absorption spectra were recorded on a Unico 4802 ultraviolet-visible spectrophotometer at room temperature. The calibration curves for biothiols were established according to the decrease of absorbance defined as ΔA=A0﹣A, where A0 and A denote the absorbance at 652 nm without and with analyte, individually.	cysteine	Color	0.5-20	μM	0.4	μM	95.9±2.7
5073	166	CB-CQDs	The detection of biothiols was performed as follows: in a series of colorimetric tubes, 0.5 mL of TMB (20 mM), 0.5 mL of H2O2 (25 mM), and 0.1 mL of CB-CQDs were fully mixed in 3.8 mL of HAc-NaAc buffer at pH4.5. Then, various concentrations of biothiols standard solution (0.1 mL) were added into the above mixture. After they were well mixed and incubated at 40 °C for 25 min, the absorption spectra were recorded on a Unico 4802 ultraviolet-visible spectrophotometer at room temperature. The calibration curves for biothiols were established according to the decrease of absorbance defined as ΔA=A0﹣A, where A0 and A denote the absorbance at 652 nm without and with analyte, individually.	cysteine	Color	0.5-20	μM	0.4	μM	95.9±2.7	105.7±2.0； 109.3±1.1； 99.7±4.3； 91.5±1.0； 98.2±2.3