Dual modality sensor using liposome-based signal amplification technique for ultrasensitive norovirus detection

References

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165Dual modality sensor using liposome-based signal amplification technique for ultrasensitive norovirus detectionhttps://doi.org/10.1016/j.bios.2020.112169Metal oxideV2O5 nanoparticles-encapsulated liposomes (VONP-LPs)

Materials

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165VONP-LPs251.5nmTEMthe particle size distribution of V2O5 NPs in the range of 15–40 nm with average lateral size of 25 � 1.5 nm.

Kinetics

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165VONP-LPsPODH2O20.04mM20.9-1μM/min165M-1 s-1
165VONP-LPsPODTMB0.02mM19.7-1μM/min165M-1 s-1165

Applications

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165VONP-LPsTo determine the linear range and sensitivity of the developed dualmodality sensor, different concentrations of NoV-LPs are examined. Anti-NoV antibody-conjugated VONP-LPs, MNPs and aliquot of NoV-LPs with various concentrations are mixed. VONP-LPs and the MNPs are bound with NoV-LPs through the specific interaction with antibody on their surface and a nanoconjugate of VONP-LPs, NoV-LPs and MNPs is formed.NoV-LPsColor10-108fg/ml4.1fg/ml
165VONP-LPsAbove results confirmed the ultra sensitivity and excellent specificity of the VONP-LPs based dual-modality biosensor proving applicability of developed sensor for real samples (Fig. 5b). To confirm the practicability real clinical samples are examined. Different types of clinical NoV (GII. 2, GII. 3, GII.4) from human feces of infected patients are detected using the developed dual-modality sensorNoV-LPs and clinical samplesColor100-107copies/ml72copies/ml