Coupling p-Hydroxybenzoate Hydroaxylase with the Photoresponsive Nanozyme for Universal Dehydrogenase-Based Bioassays

References

ref title DOI material type comment
4334 1149 Coupling p-Hydroxybenzoate Hydroaxylase with the Photoresponsive Nanozyme for Universal Dehydrogenase-Based Bioassays https://doi.org/10.1016/j.snb.2020.128859 Composite DHB coordinated SrTiO3 (SrTiO3/DHB) nanosheets

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
8200 1149 SrTiO3/DHB 50 nm TEM The synthesized SrTiO3 is a cubic phase crystal (JCPDS no. 01-079-0176) with sheet-like morphology having size around 50 nm, as confirmed by the X-ray diffraction (XRD) pattern and the transmission electron microscopy (TEM) image (Fig. 1).

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
7112 1149 SrTiO3/DHB POD TMB 4.0 40 0.22 mM 159 nM/s 1149

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
6047 1149 SrTiO3/DHB Amplified detection of G-6-P and G6PD G-6-P Color 0.05–100 μM 0.016 μM 99.3%-103.0% G-6-P in spiked serum sample
6046 1149 SrTiO3/DHB Amplified detection of G-6-P and G6PD G6PD Color 1.4 × 10–5–14 U/mL 5.7 × 10–6 U/mL