Electrochemical biosensor for ultrasensitive exosomal miRNA analysis by cascade primer exchange reaction and MOF@ Pt@ MOF nanozyme

References

ref title DOI material type comment
3709 522 Electrochemical biosensor for ultrasensitive exosomal miRNA analysis by cascade primer exchange reaction and MOF@ Pt@ MOF nanozyme https://doi.org/10.1016/j.bios.2020.112554 Composite the nanozyme could act as a signal probe (MIL-88@Pt@MIL-88@sDNA)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7753 522 MIL-88@Pt@MIL-88@sDNA 250 nm TEM After growing MIL-88 shell, the Pt NPs anchored at the MOF core seem to be covered and the size of obtained composites increase to about 250 nm (Fig. 1E and F), 76.9640 m2 g−1 and 0.2606 cm3 g−1
7752 522 MIL-88@Pt@MIL-88@sDNA 40-60 nm SEM The size distribution of CeO2 NPs ranged from 40 to 60 nm, which corresponds well with the results expected from a synthesis using with ZIF-8 NPs.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment
6385 522 MIL-88@Pt@MIL-88@sDNA TMB 522 522

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5464 522 MIL-88@Pt@MIL-88@sDNA exosomal miRNAs detection exosomal miRNAs E-chem 1 fM to 1 nM 2.00 fM