A novel nanoprobe based on core–shell Au@ Pt@ mesoporous SiO2 nanozyme with enhanced activity and stability for mumps virus diagnosis

References

ref title DOI material type comment
3915 729 A novel nanoprobe based on core–shell Au@ Pt@ mesoporous SiO2 nanozyme with enhanced activity and stability for mumps virus diagnosis https://doi.org/10.3389/fchem.2020.00463 Composite Au@Pt@mesoporous SiO2 nanozyme (APMSN), antigens-conjugated APMSN (Ags-APMSN)

Materials

ref material size size err size unit size type size comment BET b nanozyme b 10n b unit specific act sa 10n sa unit comment
7939 729 Ags-APMSNs 126.9 1.5 nm DLS Pt nanodots with sizes of 2–3 nm formed a nanoisland shell on Au NRs from the transmission electron microscopy (TEM) image (Figure 2B). The average thickness of the mesoporous SiO2 layer surrounding the Au@Pt NR was around 25 nm (Figure 2C). The DLS results revealed that the effective diameter of Au NRs, Au@Pt NRs, and APMSNs with CTAB template were 17.1 ± 0.6, 46.0 ± 0.5, and 100.6 ± 0.7 nm, respectively. Table 1 further showed that the effective diameter of the APMSNs increased evidently from 92.1 to 126.9 nm after antigen conjugation process.

Kinetics

ref material enzyme type substrate pH T km km err km 10n km unit vmax vmax err vmax 10n vmax unit kcat kcat err kcat 10n kcat unit kcat/km kcat/km 10n kcat/km unit comment

Applications

ref material application target method linear range linear ran unit LOD lod unit recovery comment
5652 729 Ags-APMSNs Mumps Virus Diagnosis mumps-specific IgM antibodies Color 10-100000 ng/mL 10 ng/mL